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trp1 pas2 1 vector  (TaKaRa)


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    Structured Review

    TaKaRa trp1 pas2 1 vector
    Trp1 Pas2 1 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trp1+pas2+1+vector/pmc00084039-139-14-41
    Average 86 stars, based on 1 article reviews
    trp1 pas2 1 vector - by Bioz Stars, 2026-09
    86/100 stars

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    Article Title: Structural and Functional Analysis of Interferon Regulatory Factor 3: Localization of the Transactivation and Autoinhibitory Domains
    Article Snippet: To delineate the transactivation domain, we subcloned different segments of on June 21, 2016 by guest http://m cb.asm .org/ D ow nloaded from IRF-3 cDNA into the TRP1 pAS2-1 vector in frame with the GAL4 DBD and tested chimeric GAL4–IRF-3 proteins for the ability to activate transcription of GAL1-lacZ reporter in yeast strain Y190 (Clontech).

    Article Title: Structural and Functional Analysis of Interferon Regulatory Factor 3: Localization of the Transactivation and Autoinhibitory Domains
    Article Snippet: To delineate the transactivation domain, we subcloned different segments of IRF-3 cDNA into the TRP1 pAS2-1 vector in frame with the GAL4 DBD and tested chimeric GAL4–IRF-3 proteins for the ability to activate transcription of GAL1-lacZ reporter in yeast strain Y190 (Clontech).

    Plasmid Preparation:

    Article Title: Structural and Functional Analysis of Interferon Regulatory Factor 3: Localization of the Transactivation and Autoinhibitory Domains
    Article Snippet: .. The wild-type and mutated forms of IRF-3 cDNA were subcloned from IRF-3/CMVBL plasmids ( 18 ) into the TRP1 pAS2-1 vector (Clontech) in frame with the GAL4 DBD or into the LEU2 pACT2 vector (Clontech) in frame with the GAL4 transactivation domain. .. For yeast one-hybrid analysis, the GAL4–IRF-3 chimeras (in the pAS2-1 vector) were transformed into Saccharomyces cerevisiae Y190 ( MATα ura3-52 his3-200 ade2-101 trp1-901 leu2-3,112 gal4Δ, met − gal80Δ URA3 :: GAL1 UAS - GAL1 TATA - lacZ ) (Clontech) by the lithium acetate permeabilization method and selected for tryptophan prototrophy according to the Matchmaker protocol.



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    TaKaRa trp1 pas2 1 vector
    Trp1 Pas2 1 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/trp1+pas2+1+vector/pmc00084039-139-14-41
    Average 86 stars, based on 1 article reviews
    trp1 pas2 1 vector - by Bioz Stars, 2026-09
    86/100 stars
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